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yepd agar plates  (A&A Biotechnology)

 
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    A&A Biotechnology yepd agar plates
    Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium <t>YEPD</t> at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).
    Yepd Agar Plates, supplied by A&A Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yepd+agar+plates/bio_rxiv__2024__07__29__605614-195-17-20?v=A%26A+Biotechnology
    Average 90 stars, based on 1 article reviews
    yepd agar plates - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "On the mode of anti- C. albicans activity of a bis(benzoxaborole) analogue of Tavaborole"

    Article Title: On the mode of anti- C. albicans activity of a bis(benzoxaborole) analogue of Tavaborole

    Journal: bioRxiv

    doi: 10.1101/2024.07.29.605614

    Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium YEPD at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).
    Figure Legend Snippet: Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium YEPD at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).

    Techniques Used: Activity Assay, Suspension, Incubation, Control



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    Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium YEPD at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).

    Journal: bioRxiv

    Article Title: On the mode of anti- C. albicans activity of a bis(benzoxaborole) analogue of Tavaborole

    doi: 10.1101/2024.07.29.605614

    Figure Lengend Snippet: Candida albicans metabolic activity reduction by benzoxaborole derivatives Influence of bezoxaboroles concentrations on metabolic activity of blastoconidial cells. MTS test (3- (4.5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, USA) was used to assess C. albicans proliferation. ( A ) The suspension of C. albicans cells in medium YEPD at the density of 1-3 x 10 2 CFU/mL was incubated with compounds 2 and Tavaborole at the range of conc.: 0.25-256 μg/mL for 48 h at 37 ° C. ( B ) The activity of benzoxaboroles against C. albicans growing in a medium with exogenous ergosterol (exErg) at 400 µg/mL for 48 h. %viable cells = OD490 test sample/OD490 control x 100%, control means cells treated with 0.96% DMSO. The results from 3 independent experiments were analyzed. Statistically significant differences were noted between non-treated growth and treated samples (P< 0.05, Unpaired t-test).

    Article Snippet: [ ] Appropriately, after 10-fold dilution series 100 μL aliquots from microtiter plates were used to inoculate YEPD agar plates (A&A Biotechnology, Poland) and after 48h incubation at 37°C the grown colonies were counted.

    Techniques: Activity Assay, Suspension, Incubation, Control